Development of EST-SSR markers for population genetic diversity assessment of Astilboides tabularis
Eui-kwon Jungp1, Bo-yun Kim2, Young-Dong Kim1
1Department of Life Science, Hallym University, Chuncheon, Gangwon-do, 24252 S. Korea; 2Multidisciplinary Genome Institute, Hallym University, Chuncheon, Gangwon-do, 24252 S. Korea
Astilboides tabularis (Hemsl) Engl. (Saxifragaceae) is a perennial herb growing in cool temperate region of NE Asia. It is a protected wild plant classified as endangered wildlife grade Ⅱ of the Ministry of Environment due to the possibility of extinction of population and/or reduction in the number of individuals by climate change. A preliminary study was conducted to develop molecular markers that can be used to compare and evaluate the genetic diversity of domestic and overseas populations of Astilboides tabularis. We first developed a total of 96 EST-SSR markers using the information from the transcriptome sequence data. We then applied these markers to a total of 40 individuals representing one Korean population (20 individuals; Bonghwachi, Gangwon-do) and one Chinese population (20 individuals; Jilin Province). As a result, 26 polymorphic markers were finally selected and a total of 78 alleles were amplified in the range of 217 bp to 276 bp. Two to five alleles were amplified in each marker (average of three alleles). The observed heterozygosity and expected heterozygosity were 0.343 and 0.272, respectively. Among the 26 EST-SSR markers, the genetic diversity (Shannon's index) of AT_029 and AT_060 were relatively high (I = 0.845 and 0.951, respectively) compared to other loci. Some of the markers amplified population specific alleles. The 26 EST-SSR markers selected in this study the population specific alleles will provide valuable basic information for assessment of genetic diversity of domestic and overseas Astilboides tabularis populations and establishment of conservation strategies for this rare species.