Ogyoeng SonP, JiYoung Jung, JeongGeun Park, GangUk Suh, Cheul-ho Lee, Sungwon Son
Plant Conservation Division, Korea National Arboretum Pochen, Korea
Astilboides tabularis (Hemsl.) Engl. is an endangered perennial herb and endemic to East Asia. The species is distributed mainly in the northern part of the Korea including Gangwon-do located in the mid-eastern part of the peninsula and Jilin and Liaoning, the northeastern provinces of China. In particular, the natural habitats of this species in Korea are known as the southern limit of its global distribution. The species is at risk of population decline caused by habitat loss and root exposure considering the characteristics in natural habitats. For these reasons, A. tabularis is now designated as endangered species in IUCN red list. We developed and analyzed microsatellite markers using NGS method (Illumian Miseq) to obtain basic data to identify and preserve the genetic diversity of A. tabularis. Using Illumina Miseq sequencing technology, we assembled 12,476,910 reads into 388,550 contigs with an average length of 294bp. Through bioinformatic analysis, the primers of 229,567 microsatellites were designed and 64 randomly chosen primer pairs were examined in 188 individuals of A. tabularis. Finally, 16 primer pairs were chosen with polymorphic alleles with consistent and successful patterns and repetitive reproducibility. Sixteen of them were polymorphic, generating a total of 176 alleles (mean alleles per locus:11). The observed and expected heterozygosities were ranged from 0.360 to 0.688, and from 0.290 to 0.670 with an average of 0.472 and 0.446, respectively. The microsatellite markers developed in this study provide a useful tool for genetic study and breeding of sequenced A. tabularis.