The genus Scapania (Dumort.) Dumort. (Scapaniaceae Mig.) is composed of about 100 taxa and are distributed in Eurasia, North America, Central America, South America, Africa, Australia, Oceania and Antarctica. The Korean Scapania is known to be distributed 15 taxa and similar to the Macrodiplophyllum and Dipolphyllum, however, this genus is distinguished from other related genera of the family by having usually strongly flattened perianths, usually elliptical to spherical gemmae and usually sharp keels. However, only the morphological studies were conducted on the Korean taxonomy, but no molecular systematic study was conducted. So, we examined 42 sequences of the cpDNA regions atpB-rbcL and psbA-trnH from Scapania (4 taxa) and four outgroup taxa to develop an efficient DNA barcode for Scapaniaceae in Korea. The degree of species resolution(72%) was significantly enhanced in combinations of the two barcode regions. Scapania formed a monophyletic grouped into each genus. However, our data showed that two DNA barcoding regions do not resolve the some taxa. Further study with more sample and DNA marker is underway to understand the phylogenetic relationship of Scapania.