The genus Hepatica Mill. is consists of 11 species in the world and distributed in the temperate regions of Northern Hemisphere. Most taxa of Hepatica grow naturally in restricted area as endemic species except some taxa. In this study, we had completed chloroplast (cp) genome sequence of ten Hepatica species. The ten Hepatica cp genome sequences ranged from 159,949bp to 161,081bp in length. In Hepatica cp genomes, 109 unique genes were annotated, including 76 protein coding genes, 29 tRNA genes and four rRNA genes. Compared with other Ranunculaceae cp genome, Hepatica cp genomes were observed several events in large single copy region. IR/LSC boundaries were expanded ca. 5.4kb in tribe Anemoneae. Also three inversions were observed. Two genes were detected as pseudogene (rps16, infA) in Hepatica. A phylogenetic analysis based on 76 protein coding genes for Ranunculaceae indicated that genus Anemone is related to genus Hepatica as a sister group and suggested that Hepatica is monophyletic in tribe Anemoneae.